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Isolation, production and characterization of amylase

Production of α-amylase from isolate Submerged fermentation was carried out using the isolate spergillus niger FAB21 for αamylase production. Mineral media as described by Singh et al. (2009) was used for the production of enzymes. The pH was adjusted to 6.5 before sterilization. Mineral media (50 mL) were prepared in 250 mL Erlenmeyer. The

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Comparative study on production of a-Amylase from

The present study deals with the production and comparative study of production of α-amylase from two strains of Bacillus licheniformis, MTCC 2617 and 2618, by using four different substrates, starch, rice, wheat and ragi powder as carbon source by submerged fermentation. The effect of varying pH and incubation temperature, activator, inhibitor, and substrate concentration was investigated on

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Amylase Production on Submerged Fermentation by Ba

Amylase Production on Submerged Fermentation by Ba Free download as File (.pdf), Text File (.txt) or read online for free. hi

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International Journal Of AMYLASE PRODUCTION ON

amylase production on solid state fermentation by wild type and mutant bacillus licheniformis & aspergillus niger from agro-wastes R.N. Shinde*, M.J. Dhangar and R.B. Narwade K. K. Wagh College of Agricultural Biotechnology Saraswati Nagar, Panchavati, Nashik 422003, Maharashtra, India

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Improved Production of Spores and Bioactive Metabolites

Improved Production of Spores and Bioactive Metabolites from Bacillus Chikindas ML, Chistyakov V (2017) Elucidation of Bacillus subtilis KATMIRA 1933 potential for spore production in submerged fermentation of (2017) Production of bioactive hydrolysate using protease, beta-glucosidase and alpha-amylase of Bacillus spp. isolated

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International Journal of Science Research (IJSR), Online

Abstract Thermostable amylase secreting bacterium was isolated through a high temperature screening procedure. The choice isolate, identified as Bacillus spp., appeared to be a facultative thermophile. Production of crude enzyme by the isolate was better when the medium was supplemented with organic nitrogen sources than the inorganic ones.

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Comparative analysis on immobilized cells of Aspergillus

Submerged fermentation B. cereus was freshly inoculated to nutrient broth and incubated in a shaker at 30°C. Medium for bacterial amylase production by submerged fermentation consisted of 6g bacteriological peptone, 0.5g MgSO 47H 2O, 0.5g KCl and 1g starch in 1000 ml distilled water. The media was prepared and distributed in 30 to 50

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n dP e iat ashmi and Gayathri, Matern ediatr utr 2017, 31

from Bacillus spp. Spectrophotometric assay of Amylase by DNS methodThe amylase was produced by submerged fermentation in the medium containing lactose 40 gl-1, yeast extract 20 gl, KH2PO4 0.05 gl, MnCl2 .4H2O 0.015 gl-1, MgSO4.7H2O 0.25 gl, CaCl2.2H2O 0.05 gl-1, FeSO4.7H2O 0.01 gl-l [25]. 100 mL of autoclaved production

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XYLANASE PRODUCTION BY A NEW Bacillus pumilus SY30A

spp. enzymes contribute about 50% of the total enzyme market. For the production of any industrial enzyme, an inexpensive substrate and an efficient fermentation process are essential for commercial viability. It is well accepted that solid-state fermentation has several advantages over submerged fermentation. Generally,

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ISOLATION AND IDENTIFICATION OF BACILLUS SP. PRODUCING

Isolation and Identification of Bacillus Sp. Producing of Amylase from Different Sources in Kirkuk, Iraq 1869 culture containing starch 10g, peptone 5.0g, MgSo 4, 7H 2 O 0.5g and KCl 2, 4H 2 0 0.5g in Distilled water up to1000 ml

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Amylase production LinkedIn SlideShare

Amylase production 1. Amylase Production on Submerged Fermentation by Bacillus spp. Made by- Miss Karishma Gangwani MSc. Biotechnology sem II Hislop School Of Biotechnology 2. What Are Amylases ? enzymes that break down starch or glycogen.

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Scale-up thermostable a-amylase production in lab-scale

a-Amylase production in shake flask a-amylase production under submerged fermentation (SmF) condition was carried out in 250 mL Erlenmeyer flasks by taking 100 mL of optimized fermentation me-dium consisting of (g/L) rice husk 10, tryptone 10, tryp topohan 1,

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Factors affecting alfa Amylase Production on

INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Factors affecting alfa Amylase Production on Submerged Fermentation by Bacillus sp. Pushpendra Singh1*, Paras Gupta2, Ravindra Singh1 and Rajesh Sharma3 1, (U.P.) India Abstract The production of extracellular amylase by Bacillus spp was optimized in a submerged fermentation.

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Enzymes & their Production LinkedIn SlideShare

Enzymes & their Production 1. Enzymes M.Sc. Biotechnology Part II (Sem III) Paper Submerged Culture Fermentation equipment used is the same as in the manufacture of (ascomycetes and basidiomycetes) and bacteria (Bacillus) Because it can act anywhere on the substrate, α-amylase tends to be faster-acting than β- amylase

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Fermentation Services at Celignis Celignis Bioprocess

Production of amylase under submerged fermentation Bacillus sp. was investigated using wheat bran, soybean meal and CaCO3 (WSC) medium. Response surface methodology (RSM) was used to evaluate the effect of the main variables, i.e., pH (11.35), temperature (35.16°C) and inoculum size (2.95%) on amylase production by applying a full factorial central composite design (CCD).

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Purification and Characterization of α-Amylase from

Enzymes from genus Bacillus spp such as Bacillus subtilis, Bacillus stearothermophilus, Bacillus licheniformis and Bacillus amyloliquefaciens are of special interest in our industrial applications as the sole producer of amylase, its commercial production and ability to withstand high temperatures [7,12]. However, the high activity of exhibited by this enzyme produced from Bacillus subtilis

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Comparative study on production of α-Amylase from

In submerged fermentation, the production of the α-amylase by the B.licheniformis MTCC strain 2617, using four different substrates as carbon source, reached maximum on the third day (Starch 2.59 IU/ml/minutes, Rice powder 2.4 IU/ml/minutes, Wheat powder 2.23 IU/ml/minutes, Ragi powder 0.89 IU/ml/minutes) and the production declined by reaching the minimum on the fifth day (Starch 1.92

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1. Production of Alpha Amylase by Bacillus cereus in

Microgen Bacillus identification kit) were all Bacillus cereus and SB2 had the largest zone of hydrolysis of 12mm on nutrient agar supplemented with starch as well as the highest enzyme activity of 1.62U/ml. Amylase activity of 2.56U/ml was obtained after 24 hours incubation in submerged fermentation. When amylase enzyme production

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EFFECT OF DIFFERENT CARBON AND NITROGEN

ABSTRACT. Amylases are among the most important enzymes in present-day biotechnology. The effects of various carbon and nitrogen sources on α-amylase production by submerged fermentation (smF) from Bacillus amyloliquefaciens was investigated. The study revealed that the levels of the α- amylase production varied greatly with the type and concentration of carbon and nitrogen sources used.

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Bacterial α Amylase Production UK Essays

A Bacillus strain has been subjected to manipulation following the principles of genetic engineering and plasmid expression vectors such as coliphage 105 for enhanced alpha amylase production [4]. The gene coding for the protein of interest is interpolated under the

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Comparative study on production of a-Amylase from

In submerged fermentation, the production of the α-amylase by the B.licheniformis MTCC strain 2617, using four different substrates as carbon source, reached maximum on the third day (Starch 2.59 IU/ml/minutes, Rice powder 2.4 IU/ml/minutes, Wheat powder 2.23 IU/ml/minutes, Ragi powder 0.89 IU/ml/minutes) and the production declined by reaching the minimum on the fifth day (Starch 1.92

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Isolation, production and characterization of amylase

Production of α-amylase from isolate Submerged fermentation was carried out using the isolate spergillus niger FAB21 for αamylase production. Mineral media as described by Singh et al. (2009) was used for the production of enzymes. The pH was adjusted to 6.5 before sterilization. Mineral media (50 mL) were prepared in 250 mL Erlenmeyer.

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() SCREENING OF POTENTIAL ISOLATE FOR THE

Amylolytic bacteria were isolated from decayed pomegranate. The bacteria were screened for amylase activity using starch agar plate assay. Five different substrates were used namely banana peel, Gossypium oil cake, groundnut oil cake, cassava, and

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Soybean meal through solid-state fermentation Feed

Benefits of solid-state fermentation versus submerged fermentation. Research by Soares et al. compared solid-state fermentation (SSF) with submerged fermentation (SF) and protease production of Bacillus subtilis.They found that the enzyme concentration was 10-fold greater and enzyme productivity was 45 percent higher than the protease produced from SF (Soares VF, 2005).

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Solid State Fermentation of a Raw Starch Digesting

Do?ru, and H. Alkan, "Production of extracellular alkaline alpha-amylase by solid state fermentation with a newly isolated Bacillus sp," Preparative Biochemistry & Biotechnology, vol. 38, no. 2, pp. 184190, 2008.

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Amylase Production on Submerged Fermentation by Ba

Amylase Production on Submerged Fermentation by Ba Free download as File (.pdf), Text File (.txt) or read online for free. hi

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CONCOMITANT PRODUCTION OF TWO PROTEASES AND ALPHA- AMYLASE

1073 Mukhtar, H. et al. Production of proteases and alpha-amylase by B. subtilis Steptomyces spp., Themomyces lanuginosus, Rhizopus spp., Trichoderma spp., Penicillium spp. Fusarium spp. and Aspergillus spp. (7, 8, 19, 1). Proteases and alpha amylases occupy a pivotal position with respect to their applications in industrial fields and hence

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Bacterial Amylase A Review ijpbafo

Bacillus . spp., Bacillus licheniformis, and . Bacillus halodurans [27]. These enzymes were purified and characterized. Some of them have been reported to be maltooligosaccharide producing enzymes [28]. Production of oligosaccharides with the enzymes is attractive because they are more stable than general amylase showing high activity at a region of 3040°C. The enzyme was not suitable for

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Production, characterization and application of

Alkaline amylase production by submerged fermentation (SmF) 16S rRNA gene sequences of CH11 and its homologs which includes seven Bacillus spp. and one uncultured Planctomycetes Sva0503 as an outgroup were taken for Multiple Sequence Alignment.

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Production, purification and enzyme kinetics of amylase

Production, purification and enzyme kinetics of amylase from bacteria isolated from a soil of solapur. purification and enzyme kinetics of amylase from bacteria isolated from a soil of solapur.

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